Tadpole Brains

How we study Tadpole brains

The Xenopus Neurobiology Laboratory at the University of st Andrews uses newly hatched tadpoles of the South African Clawed Toad (Xenopus laevis) to investigate how its simple brain and nervous system produces the behaviour which enables the tadpoles to survive. This work was inspired by pioneering studies by George Coghill on the development of behaviour and the nervous system in hatchling Tiger salamanders and started by the Emeritus Prof Alan Roberts in the university of Bristol.

To study nerve cell activity and connections the tadpoles have to be immobilised using the snake venom: alpha-bungarotoxin. We then make electrical recordings from some of the motor nerves that normally make the swimming muscles contract. When we touch the tadpole, it does not move, but rhythmic activity in the motor nerves show that it has started to swim. If we press on the head, to imitate the tadpole bumping into something,  swimming activity stops. These responses are just like the behaving tadpole.

To find out what is happening inside the nervous system the most important method we use is to make electrical recordings from one or two individual nerve cells in the brain or spinal cord.  The recording electrodes can be used to inject dye so, after the experiment, we can see what the nerve cells look like.

More coming soon!

The Digestive System

 

The digestive system is modified to account for the change of the herbivorous diet of the tadpole to the carnivorous diet of the frog.

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The Skin

 

The skin adapts for the change from a purely aquatic lifestyle to an amphibious lifestyle.

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The Reproductive System

 

The urogenital system develops to allow for reproduction in adulthood.

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The Skeleton

 

The skeletal structure develops to accommodate the change from tail swimming to using legs to move around. The skull also needs to be remodelled for a frog's change in vision.

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The Nervous System

 

A tadpole sees from eyes that are positioned on opposite sides of the head. During metamorphosis, the optical nerves develop to accommodate a frog's binocular vision, where the eyes are positioned at the front of the head.

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1. Mating and Laying Spawn

 

Male and female frogs go to ponds in the winter. They mate in the spring, and the female lays big clumps of eggs.

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2. Frog Spawn

 

Frogs eggs are called frogspawn. Each round black egg is about 1 mm wide and is surrounded by a blob of jelly. Other animals produce spawn as well, which you can look at here.

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3. Maturing Frog Spawn

 

After a few days, the eggs begin to grow into tiny tadpoles inside the jelly.

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4. Hatchlings

 

Then the tadpoles hatch! They are about 5 mm long and they can’t swim (yet). They can bend their body from side to side using special muscles along their trunks and tails.

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5. Young Tadpoles

 

When their tail is big enough, they swim off into the pond to start to feed. At first they have gills (the pale protrusions from the head region in the left photo) so they can breath underwater like fish. Young tadpoles feed by grazing the surface of pond weeds and also eating tiny floating plants called algae.

Click here to play a tadpole feeding game called Taddypole!

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6. Maturing Tadpoles

 

Later they develop lungs and can swim up to the surface of the water to breath. The gills are absorbed back into their bodies and eyes develop. Older tadpoles are then able to feed on small animals like young insects.

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7. Mature Tadpoles

The next stage in development is to grow back legs. Tadpoles during this stage need to eat meat in order to get the proper nutrients to grow.

If you are looking after tadpoles, be careful as they can eat each other if you don't give them meat to eat! Click here to learn more about how to look after tadpoles as pets.

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8. Froglet

Finally, tadpoles grow front legs and their tail shrinks until it almost disappears. This is when they climb out of the pond and start living on dry land. Small frogs are commonly called froglets.

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9. Adult Frog

The little froglets will stay by the pond and slowly grow over the summer, eating small insects and worms. They will hibernate just like other adult frogs in damp spots near ponds from autumn until the next spring.

After four years, the new frogs will become adults and will be ready to mate and begin the cycle again.

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Copy - 9. Adult Frog

The little froglets will stay by the pond and slowly grow over the summer, eating small insects and worms. They will hibernate just like other adult frogs in damp spots near ponds from autumn until the next spring.

After four years, the new frogs will become adults and will be ready to mate and begin the cycle again.

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Desert Habitats

Desert habitats are the driest habitats in the world. Most people only think of very hot habitats as being deserts, but cold habitats can be deserts as well! Animals and plants that live in deserts have the ability to survive on very little water and animals can control their body temperatures so they stay at the right level.

 Some examples of plants and animals that live in deserts are cacti, the desert tortoise and the artic fox.

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